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J Korean Soc Environ Eng > Volume 41(9); 2019 > Article
J Korean Soc Environ Eng 2019;41(9): 494-500. doi: https://doi.org/10.4491/KSEE.2019.41.9.494
지질추출 미세조류 폐기물로부터 바이오 수소 생산 향상을 위한 전처리의 적용
김태훈1, 임병서2, 박혜숙3, 윤여명1
1충북대학교 환경공학과
2한국환경공단 자원재활용처
3대전보건대학 환경보건과
Enhanced Bio-hydrogen Production from Pretreated Microalgal Waste
Tae-Hoon Kim1, Byung-Seo Lim2, Hye-Sook Park3, Yeo-Myeong Yun1
1Department of Environmental Engineering, Chungbuk National University
2Department of Resources Recycling, Korea Environment Corporation
3Daejeon Health Institute of Technology
Corresponding author  Hye-Sook Park ,Tel: 042-670-9223, Fax: 042-670-9525, Email: hspark@hit.ac.kr
Yeo-Myeong Yun ,Tel: 043-261-2466, Fax: 043-264-2465, Email: ymyun@cbnu.ac.kr
Received: August 7, 2019;  Revised: September 1, 2019;  Accepted: September 2, 2019.  Published online: September 30, 2019.
Objectives : This study was performed to investigate optimal conditions for fermentative hydrogen production of lipid-extracted microalgae waste (LMW) by single and combined pretreatments. Methods : To produce LMW as a feedstock, the lipids were extracted from Chlorella vulgaris by conventional method and the LMW concentration was controlled at 75 g dry cell weight (dcw)/L. LMW pretreatment was performed by single (alkali, acid, and ultrasonic (U/S)) and combined (alkali+ultrasonic, acid+ultrasonic) pretreatment. For performing dark fermentative hydrogen production, LMW was added on the basis of a solid/liquid ratio (S/L ratio) of 1:10 and the heat-treated sludge at 90℃ for 20 min was inoculated in batch reactors. After the batch experiment, microbial community analysis was performed by Next Generation Sequencing (NGS).
and Discussion : The acid pretreatment showed the greatest influence on the increase of the solubilization rate among three single pretreatment methods. On the other hand, the ultrasonication pretreatment did not lead to increasing the solubilization but showed increased hydrogen production (197 mL H2/g (dcw)) as much as the single acid pretreated methods. The combined pretreatment resulted in the highest 6,880 mg COD/L in the sample pretreated by the acid (1.5% HCl) combined with the single ultrasonic method, but the highest hydrogen production of 237 mL H2/g (dcw) was obtained in the sample pretreated by the acid (1.0% HCl) combined with ultrasonic. However, the alkali combined with ultrasonic combined pretreatment method showed a slight increase in hydrogen production compared to control one. Microbial community analysis revealed that the relative abundance of genera Clostridium was 97.1% in the sample pretreated by combined pretreatment (1.5% HCl+ultrasonic).
: The improvement of hydrogen production was closely related to the increase of SCOD in both samples employed by single and combined pretreatment. Especially, the application of acid pretreatment method was found to be more effective than alkali pretreatment. Ultrasonic pretreatment, on the other hand, did not result in increased SCOD, but improved hydrogen production. This might be due to that physical structure of LMW was disrupted and became easier biodegradable, even if the solubilization of the substrate did not proceed through the pretreatment of the ultrasonic method. According to the result of NGS analysis, high abundance of Clostridium spp. played the main role in hydrogen production.
Key Words: Hydrogen, Lipid-extracted Microalgae Waste, Next Generation Sequencing, Pretreatment
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