Variation of Nitrogen Removal Efficiency and Microbial Communities Depending on Operating Conditions of a CANON Process

Jo, Kyungmin; Park, Younghyun; Cho, Sunja; Lee, Taeho; Jo, Kyungmin; Park, Younghyun; Cho, Sunja; Lee, Taeho

doi:2015.37.6.332

The Korean text of this paper can be translated into multiple languages on the website of http://jksee.or.kr through Google Translator.

J Korean Soc Environ Eng > Volume 37(6); 2015 > Article

Original Paper

J Korean Soc Environ Eng 2015;37(6): 332-339. doi: https://doi.org/10.4491/KSEE.2015.37.6.332

CANON 공정에서 운전조건에 따른 질소 제거효율 및 미생물군집 변화

조경민¹, 박영현¹, 조순자², 이태호¹

¹부산대학교 사회환경시스템공학부
²부산대학교 미생물학과

Variation of Nitrogen Removal Efficiency and Microbial Communities Depending on Operating Conditions of a CANON Process

Kyungmin Jo¹, Younghyun Park¹, Sunja Cho², Taeho Lee¹

¹Department of civil and environmental engineering, Pusan National University
²Department of microbiology, Pusan National University

Corresponding author

Taeho Lee ,Tel: 051-510-2465 , Fax: 051-514-9574, Email: leeth55@pusan.ac.kr

Received: April 3, 2015; Revised: June 15, 2015; Accepted: June 20, 2015. Published online: June 30, 2015.

ABSTRACT

Nitrogen removal is one of the most important issues about wastewater treatment because nitrogen is a primary pollutant caused various problems such as eutrophication. We developed a CANON microbial community by using AOB and ANAMMOX bacteria as seeding sources. When 100 mg-N/L of influent ammonium was supplied, the DO above 0.4 mg/L showed a very low TN removal efficiency while the DO of 0.3 mg/L showed TN removal efficiency as high as 71.3%. When the influent ammonium concentration was reduced to 50 mg/L, TN removal efficiency drastically deceased. However, TN removal efficiency was recovered to above 70% after 14 day operation when the influent nitrogen concentration was changed again from 50 mg-N/L to 100 mg-N/L. According to the operating temperature from 37±1℃ to 20±1℃, TN removal efficiency also rapidly decreased but gradually increased again up to 70.0±2.6%. The analysis of PCR-DGGE showed no substantial difference in microbial community structures under different operational conditions. This suggests that if CANON sludge is once successfully developed from a mixture of AOB and ANAMMOX bacteria, the microbial community can be stably maintained regardless of the changes in operational conditions.

Key Words: CANON, ANAMMOX, Partial Nitrification, Microbial Community